A 16S rDNA-based nested PCR protocol to detect Campylobacter gracilis in oral infections
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Campylobacter gracilis and Campylobacter rectus in primary endodontic infections.
AIM A species-specific nested polymerase chain reaction (PCR) assay was used to investigate the occurrence of Campylobacter gracilis and C. rectus in primary root canal infections. METHODOLOGY Samples were collected from 57 single-rooted teeth with carious lesions, necrotic pulps and radiographic evidence of periradicular disease. Twenty-eight cases were diagnosed as chronic asymptomatic peri...
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Patel A, et al. Arch Dis Child Educ Pract Ed 2017;0:1–4. doi:10.1136/archdischild-2016-312049 INTRODUCTION PCRs have revolutionised the detection of bacteria in clinical samples since their widespread introduction in the 1990s. Quantitative PCR (qPCR), also known as specific PCR, involves the targeting of particular bacterial species. The technique uses specific primers (short strands of nuclei...
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Check out our Selection Guide! PCR RNA DNA LAMP KitsOne Tube For Superior Reproducibility. From fish tissues or fluids using a nested PCR primer set. The use of trade, firm, or corporation names in this protocol is for the information and.jor consideration in designing a nested PCR protocol compatible with quantitation is to assure that the maximum concentration of PCR products produced by the....
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DNA corresponding to 16S rDNA and the 165-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558T and Escherichia coli ATCC 11775T were included as outgroups. PCR-amplified products ...
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Nocardiosis is an uncommon infection caused by the aerobic actinomycete nocardia. Identification of the pathogen is essential for the definitive diagnosis and for an effective treatment. This report describes a case of chronic granulomatous pleuritis caused by nocardia. A 59 year old Japanese man had a history of repeated pyothorax. Right pleural decortication and thoracic drainage were perform...
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ژورنال
عنوان ژورنال: Pesquisa Odontológica Brasileira
سال: 2003
ISSN: 1517-7491
DOI: 10.1590/s1517-74912003000200008